Figure 2
Proposed reaction steps in the conversion of PUT to HSP by bacterial HSS. The relevant residues, NAD(H), PUT, HSP and intermediates are shown as two-dimensional structure representations. Hydrogen bonds are depicted as blue dotted lines, delocalized electrons as dashed lines, cation–π interactions as orange dashed–dotted lines and electron transfers as red arrows. Atom numbering for PUT and HSP is given in green. For simplicity, steps 5 and 6 are shown as a combined depiction with correspondingly labeled electron transfers. A more detailed sequence of the reaction steps has been described previously (Krossa et al., 2016) and additional intervening reaction steps are proposed in Supplementary Fig. S2. |