Figure 8
BvHSS variants targeting the `ionic slide' and the `inner amino site'. (a) BvHSS variant E117Q (PDB entry 6s6g chain B), (b) superimposition of wild-type BvHSS (PDB entry 4tvb chain B, gray) onto (a), (c) the E210Q variant (PDB entry 6s3x chain A) and (d) the E210A variant (PDB entry 6s49 chain A). Atomic models are depicted in ball-and-stick representation and electron-density maps as mesh. The large images (a, c, d) show the 2mFo − DFc maps (black, 1σ) and mFo − DFc maps (green/red, ±4σ) of the complete models. Models lacking the respective exchanged residue together with ten N-terminally and ten C-terminally adjacent residues were used to calculate (I) 2mFo − DFc omit maps (black, 1σ) and (II) mFo − DFc omit maps (green/red, ±4σ) after refinement including simulated annealing. |