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Figure 3
Dimeric structures of SdgB and SdgA. (a, b) The dimeric structures of SdgB (a) and SdgA (b) are presented as cylindrical models in surface view, and each monomer is colored sky and orange. The core domains are labeled, and the expected key sites and dimerization area are marked with orange and gray dotted lines, respectively. (c, d) The distribution of the sedimentation coefficient of SdgB (c) and SdgA (d) [c(s) versus s, where s is in svedbergs (S)] from the sedimentation-velocity experiments. (e) SPR analyses for measurement of the binding affinity between SdgB and SdgA. The SPR sensorgram shows the direct binding of SdgA (0.391–25.0 µM) to immobilized SdgB. The calculated ka, kb and Kd values are also shown below the plot.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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