Figure 6
The putative binding paths for the negatively charged SDR substrates in the SdgB and SdgA structures. (a) The binding mode of multiple phosphate ions in the SdgBphosphate structure. SdgBphosphate is superimposed onto SdgBquaternary and the phosphate ions are shown as sticks and spheres with a 2mFo − DFc electron-density map contoured at 1.0σ as a cyan mesh. The electrostatic surface model is presented using SdgBquaternary. (b, c) Charged surface views of the ABD of SdgBquarternary (b) and SdgAunbound (c). The representative residues constituting the surface are labeled. The SD-repeat peptide and phosphates complexed in SdgBquaternary and SdgBphosphate, respectively, which are superimposed into SdgAunbound, are presented in stick models for comparison. |