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Figure 1
Binding of selected metals to apo PA4043. Analysis by ITC measurements was performed by titrating 11 µM PA4063 with 320 µM zinc acetate (a) or cobalt nitrate (b) solution in 84% dialysis buffer (150 mM NaCl, 20 mM Tris pH 7.2). The upper panels show the raw ITC data, while the lower panels show the integrated energy values normalized for injected protein. Binding isotherms were fitted using a single-binding-site model.

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ISSN: 2059-7983
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