Figure 3
Dynamic properties of the DREP3 CIDE domain. (a) SEC chromatograms of the DREP3 CIDE domain and other CIDE domains. All SEC experiments were performed using 20 mM Tris–HCl pH 7.9, 500 mM NaCl buffer. (b) SEC chromatograms of samples of the DREP3 CIDE domain at three different concentrations. (c) SEC chromatograms of samples of the DREP3 CIDE domain at four different NaCl concentrations. The DREP3 CIDE domain formed large oligomers under low-salt conditions (50 mM) and migrated at smaller sizes with increasing salt concentration. (d) SEC-MALS measurements of various forms of the DREP3 CIDE domain in different concentrations of NaCl. The red line indicates the experimentally calculated molecular mass. |