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Figure 1
X-ray mosaics (7 × 7 frames) from areas of interest collected from two different grids bearing vitrified U2OS cells with (a) the 40 nm and (d) the 25 nm zone plate. Data were collected in the highlighted areas in (a) and (d) and representative slices from the reconstructed tomograms at different z heights can be seen in (b), (c), (e) and (f) (colour-coded to match the originating mosaic). The arrows used to highlight the cellular features are nuclear membrane in blue, outer cell membrane in purple, endoplasmic reticulum in yellow, mitochondria in orange, multivesicular bodies in green and actin bundles in white. Scale bars are 10 µm for (a) and (d) and 1 µm for (b), (c), (e) and (f). |
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