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Figure 3
2D slices of imaging data along z starting from near the support surface (a, b), followed by slices from the mid-region of the sample (d, e) and finally the upper area of the sample (g, h). SIM data are shown in (a), (d) and (g) and the corresponding SXT data are shown in (b), (e) and (h). The correlated images are shown in (c), (f) and (i). All SXT data sets shown here were collected using the 40 nm zone plate. Red arrows indicate bundles of F-actin that are barely delineated in the SXT data but can be clearly identified based on the correlation of the associated fluorescence signal. Green arrows point to cell-surface protrusion association of F-actin, while the blue arrow shows an example of local actin concentration around a 2 µm diameter vesicle. Scale bars are 1 µm. |
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