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![[Figure 2]](cb5130fig2mag.jpg)
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Figure 2
(a) Phylogenetic tree made in MEGA X based on proteins found in BlastP with a query coverage of 45% or more (obtained in September 2021) along with four structurally related enzymes found by the DALI server. (b) All protein sequences in the phylogenetic tree are displayed along with three relevant conservative domains found in the NML Conserved Domain Database (CDD). Rows 1–9 correspond to Hjc_15-6 aligned with the Hj-resolving enzyme from P. furiosus (found by the DALI server) and all phage proteins found in BlastP, except for Thermus phage phiFA. The alignment was performed in Jalview (Waterhouse et al., 2009  ) using the ClustalO web service with default settings and aligned output order, with colouring by ClustalX. In this study, the conserved residue Glu10 is suggested to complement the PD-(D/E)XK catalytic motif and the respective SD-EVK motif (found in the phage sequences except for that of Thermus phage phiFA) to build a proposed signature. The proposed signature is marked with black boxes and consists of residues E10-SD40-EVK55 in Hjc_15-6 and other aligned Hj-resolving enzymes originating from phages (rows 1–2 and rows 6–8) and residues E10-PD40-EVK55 in the conservative domains (rows 3–5). |
![[Figure 2]](cb5130fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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