Structural snapshot of a glycoside hydrolase family 8 endo-β-1,4-glucanase capturing the state after cleavage of the scissile bond

Fujiwara, T.; Fujishima, A.; Nakamura, Y.; Tajima, K.; Yao, M.

doi:10.1107/S2059798321012882

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 2
BC hydrolysis assay for EbBcsZ. (a) The state of gelatinous BC after 20 h of incubation with cellulase ONOZUKA RS, bovine serum albumin (BSA), EbBcsZ and EbBcsZ(D242A). (b) Turbidity of the assay solution measured after 20 h of incubation. Average and standard deviation values of the absorbance at 600 nm were calculated from four replicates (N = 4).

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 78| Part 2| February 2022| Pages 228-237

https://doi.org/10.1107/S2059798321012882

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