Figure 3
SpReAD analysis of diFe-SulE with data collected at different total average diffraction-weighted doses of (a) 0.26 MGy, (b) 0.57 MGy and (c) 2.70 MGy. The top panel shows the respective SpReAD profiles and the bottom panel shows an anomalous difference electron-density map (green mesh) contoured at 13σ for the data set at 7126 eV. Metal ions are shown as spheres. Residues that show multiple conformations are indicated. For clarity, Glu126 has been omitted. |