A recurring packing contact in crystals of InlB pinpoints functional binding sites in the internalin domain and the B repeat

Geerds, C.; Bleymüller, W.M.; Meyer, T.; Widmann, C.; Niemann, H.H.

doi:10.1107/S2059798322000432

Figure 4
All crystallographically independent InlB₃₉₂ molecules form a crystal contact between their B repeat and a symmetry-related IR region. B repeats are structurally aligned and colour-coded as in Fig. 1

. Symmetry-related molecules are shown in grey. (a) The same contact is formed in both crystal forms of InlB₃₉₂_T332E and in InlB₃₉₂_wt between the B repeat of chain C and the IR region of chain A. The B repeat and the symmetry-related InlB₃₉₂ are coloured as follows: InlB₃₉₂_T332E crystal form I (PDB entry 7pv8), green and medium grey; InlB₃₉₂_T332E crystal form II (PDB entry 7nms), dark green and dark grey; InlB₃₉₂_wt (PDB entry 7pv9; chains C and A), cyan and light grey. (b) A similar contact is formed in InlB₃₉₂_wt between the B repeat of chain A or B and the IR region of chain C or B, respectively. The B repeat and the symmetry-related InlB₃₉₂ are coloured as follows: chains B, blue and medium grey; chains A and C, dark blue and dark grey. (c) An overlay of the B repeats of InlB₃₉₂_wt chain A (blue) and InlB₃₉₂_T332E crystal form II (dark green) shows that the IR regions of the symmetry-related molecules are shifted. (d) Contact of the B repeat (C atoms in dark green) and a symmetry-related IR region (carbons in dark grey) of InlB₃₉₂_T332E crystal form II. 2mF_o − DF_c electron density is contoured at 1σ.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 78| Part 3| March 2022| Pages 310-320

https://doi.org/10.1107/S2059798322000432

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