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![[Figure 2]](jc5044fig2mag.jpg)
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Figure 2
(a) PACE analysis of the activity of RBcel1_WT on 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-cellotrioside (ClPNP β-BG3) with different sugar acceptors. Lanes 1–5 show the reaction products when 1 mM ClPNP β-BG3 was incubated with 10 µM enzyme for 10 min at 25°C: lane 1, without an acceptor; lane 2, with 1 mM G1; lane 3, with 1 mM G2; lane 4, with 1 mM G3; lane 5, with 1 mM G4. Lanes 7–11 show the corresponding control reaction mixtures incubated without enzyme: lane 7, ClPNP β-BG3; lane 8, ClPNP β-BG3 + G1; lane 9, ClPNP β-BG3 + G2; lane 10, ClPNP β-BG3 + G3; lane 11, ClPNP β-BG3 + G4. Lane 6: oligosaccharide size ladder comprising G1, G2, G3, G4, G5 and G6. (b) PACE analysis to validate 4,6-O-benzylidene-β-cellotrioside (BG3) as a product of the hydrolysis of ClPNP β-BG3. Lane 1, oligosaccharide size ladder comprising G1, G2, G3, G4, G5 and G6; lane 2, 1 mM BG3 incubated without enzyme; lane 3, products of the incubation of 1 mM ClPNP β-BG3 with 1 mM G3 and 10 µM enzyme; lane 4, 1 mM G4 incubated without enzyme. |
![[Figure 2]](jc5044fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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