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Figure 8
(a) Structural alignment of the four monomers of RBcel1_Y201F showing the position of the side chain of Glu245 and the ligands in the +1 to +3 subsites. Monomers A, B, C and D are represented in blue, green, pink and orange, respectively. To highlight the displacement of the side chain of Glu245 on the formation of the GEI, the apo-form structure of RBcel1_Y201F was added to the alignment (cyan; PDB entry 7p6i). G2 is found in the +1 to +2 subsites in monomers A, B and D, while G3 lies in the +1 to +3 subsites in monomer C. (b) Close-up view of the −1 and +1 subsites showing the structural changes when G3 is bound in the positively numbered subsites instead of G2. For the sake of clarity, only the superposition of monomer A (blue) with monomer C (pink) is shown. The distances between O4 of the acceptor sugar and C1 of the GEI or OE1 of Glu135 are given in the respective colors of monomers A and C.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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