view article
Figure 4
Possible carboxylate–hydroxyl relay mechanism including His124. (a) Schematic drawing of the proposed His124-dependent proton-relay pathway. The amino-acid residues, Mg2+ ions and H2Oδ− water molecule are drawn based on the current crystal structure. A substrate is drawn on the basis of the hypothetical structure in comparison with the human RNase H1–RNA/DNA hybrid complex (PDB entry 2qkk). A putative nucleophilic attack water (gray) is also indicated. (b) A putative mechanism for the formation of an intermediate ternary complex after the RNase HI–Zn2+ complex. The Zn2+-coordinated water molecule resembling that in RNH_Zn_1 and RNH_Zn_2 is indicated in the first step of the scheme.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 78| Part 3| March 2022| Pages 390-398
https://doi.org/10.1107/S2059798322000870
Follow Acta Cryst. D
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS