Fast fragment- and compound-screening pipeline at the Swiss Light Source

Kaminski, J.W.; Vera, L.; Stegmann, D.P.; Vering, J.; Eris, D.; Smith, K.M.L.; Huang, C.-Y.; Meier, N.; Steuber, J.; Wang, M.; Fritz, G.; Wojdyla, J.A.; Sharpe, M.E.

doi:10.1107/S2059798322000705

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 1
Schematic of the FFCS pipeline. Initial crystals are optimized in SWISSCI MRC-3 plates and tested for DMSO resilience. Plates are stored in Rock Imager systems at 4°C or 20°C and are imaged at regular intervals. Selected fragments are contactlessly transferred from small-volume stock solutions to preselected crystal drops with the Echo liquid-handling device. Subsequent semi-automated crystal harvesting, supported by the Crystal Shifter robot, allows the efficient freezing of soaked crystals.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 78| Part 3| March 2022| Pages 328-336

https://doi.org/10.1107/S2059798322000705

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