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![[Figure 1]](jv5008fig1mag.jpg)
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Figure 1
The experimental setup and broadening of the peak during SEC–SAXS. (a) Molecular visualization of a DMPC-loaded csMSP1D1ΔH5 nanodisc built with CHARMM-GUI NanodiscBuilder (Jo et al., 2008  ; Qi et al., 2019). (b) Schematic of the SEC–SAXS setup to reiterate the distance between the HPLC UV280 absorbance detector and the capillary where SAXS is recorded. (c) Normalized chromatogram and scattergram for csMSP1D1ΔH5 nanodiscs. The grey points indicate UV absorbance and the red points indicate the total intensity per frame. Solid lines are exponentially modified Gaussian (EMG) fits to the data. The centres of the two peaks are aligned. (d) The black profile is the EMG fit to the chromatogram in absorbance units. The red profile is the corrected version substituting in parameters from the fit to the scattergram while keeping the area under the curve constant. (e) I(0)/c as a function of the elution volume. Black points are calculated from the original SEC profile. Red points are calculated from the corrected profile. The dashed line is the theoretical value estimated for 120 DMPC per nanodisc. |
![[Figure 1]](jv5008fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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