Figure 10
Microscopic observation of a PAO1 suspension treated with Pae87 over time. (a) Fluorescence and phase-contrast images representative of the observations made during 2 h of incubation (37°C, 20 mM NaPiB pH 6.0, 150 mM NaCl) of a PAO1 suspension (∼108 CFU ml−1) treated with 10 µM Alexa488-labelled Pae87 and stained with PI. White bars indicate 10 µm. (b) Time-wise distributions of cells or aggregate areas estimated using the LAS X microscopy image-analysis software for at least ten frames per time point. A one-way ANOVA followed by a Tukey's post-test was applied for multiple comparisons. Distributions marked with different letters are significantly different from each other, while no significant differences were found between those indicated with the same letter. |