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![[Figure 5]](ni5018fig5mag.jpg)
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Figure 5
Activities of wild-type Pae87 and of mutants in which conserved catalytic site glutamic acid residues were mutated. (a) Muralytic activity of Pae87 and its mutants on RBB-labelled purified PAO1 cell walls at different concentrations. (b) Comparison of muralytic activities at the maximum concentration tested (1 µM). (c) Bactericidal activity of 10 µM Pae87 and its mutants against bacterial suspensions of PAO1 (2 h, 37°C). (d) Fluorescence microscopy of some of the treated suspensions in (c) dyed with SYTO9 and propidium iodide. White bars at the lower left corner indicate the scale (10 µm). One-way ANOVA was used in (b) and (c) followed by a Tukey post-test to perform an all-against-all multiple comparison (ns, non-significant difference; ***, p ≤ 0.01). |
![[Figure 5]](ni5018fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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