Monomodular Pseudomonas aeruginosa phage JG004 lysozyme (Pae87) contains a bacterial surface-active antimicrobial peptide-like region and a possible substrate-binding subdomain

Vázquez, R.; Seoane-Blanco, M.; Rivero-Buceta, V.; Ruiz, S.; van Raaij, M.J.; García, P.

doi:10.1107/S2059798322000936

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 8
Antimicrobial activity of P87 peptide. (a) Far-UV circular-dichroism spectra of 20 µM P87 in 20 mM NaPiB pH 6.0 with 100 mM NaCl and different (v/v) concentrations of TFE (as indicated in the legend). (b) Turbidity decrease assay of a 10 µM P87 or Pae87 treatment of PAO1 cell suspensions. (c) Bactericidal activity range of 10 µM P87. The Pae87 bactericidal range from Vázquez, Blanco-Gañán et al. (2021

) is also shown for comparison.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 78| Part 4| April 2022| Pages 435-454

https://doi.org/10.1107/S2059798322000936

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