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![[Figure 9]](ni5018fig9mag.jpg)
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Figure 9
OM permeabilization assays with the fluorescent probe NPN. (a) Generalized additive model estimation of the average tendencies of the NPN fluorescence kinetics (the excitation wavelength was 350 nm and emission was recorded at 420 nm). Estimation was based on three independent replicates; the mean estimation ± 95% confidence interval (shaded grey) is shown for each experimental condition. (b) Comparison of the average NPN signal (minus background fluorescence) after 5 min incubation. The mean ± standard deviation of three independent replicates is shown; a one-way ANOVA test with a Dunnett post-test was applied to statistically compare each condition with the control (untreated cells in the presence of the probe). *, p ≤ 0.05; ****, p ≤ 0.0001. (c) Fluorescence microscopy observation of each experimental condition after 2 h incubation. Superimpositions of phase-contrast images with blue fluorescence signal observed with an A filter cube (excitation bandpass 340–380 nm) are shown. RFU, fluorescence units relative to the maximum value achieved during the assay. |
![[Figure 9]](ni5018fig9.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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