Figure 1
Crystal structure of the ternary BPGM·3-PGA·2-PG complex. (a) Overall structure of BPGM·3-PGA·2-PG (ribbons) with 3-PGA (sticks) bound at the active site of monomer A and 2-PG (sticks) bound at the active site of monomer B and at the dimer interface. (b) Electron-density map of the active site of monomer A with bound 3-PGA (2Fo − Fc map contoured at the 0.8σ level). (c) Electron-density map of the active site of monomer B with bound 2-PG (2Fo − Fc map contoured at the 0.8σ level). (d) Detailed interactions between the active-site residues of monomer A (cyan sticks) and 3-PGA (yellow sticks). (e) Detailed interactions between the active-site residues of monomer B (pink sticks) and 2-PG (green sticks). (f) Electron-density map of the dimer interface residues with bound 2-PG (2Fo − Fc map contoured at the 0.8σ level). (g) Detailed interactions between the dimer interface residues (cyan sticks for monomer A and pink sticks for monomer B) and 2-PG (green sticks). |