Figure 2
(a) Michaelis–Menten kinetic plot for hydrolysis of pNP-Ac by PvSAE. Rates were established using a pNP standard curve. Experiments were performed in triplicate. (b) Activity of PvSAE on bovine submaxillary mucin. Acetate release from 6.25 mg ml−1 bovine submaxillary mucin was quantified after 1 h of incubation at 37°C with a buffer control or 100 nM or 1 µM PvSAE. |