Figure 3
Pentamer formation involves inter-subunit zinc binding and a salt bridge. (a) Structure-based multiple sequence alignment of Kv2.1 with KvS (Kv9.3, Kv8.2 and Kv6.4), Kv3.1, Kv4.1 and Kv1.1 T1 domains. Blue triangles below residues indicate the Zn2+-coordination motif HX5CX20CC and magenta circles indicate the negatively charged CDD motif and its interacting positive residues at the N-terminus. Shading of residues in blue or magenta, respectively, highlights conservation. (b) Cartoon representation of Kv2.1 T1 with residues at the subunit interface highlighted as sticks; colors are chain A in green and chain B in orange. The insets show the interacting residues in the Zn2+-coordination site (left) or CDD motif (right). Hydrogen bonds are shown as black dashed lines, the zinc ion is in gray, O atoms are in red, S atoms are in yellow and N atoms are in dark blue. |