Figure 5
Negative-stain transmission electron microscopy of Kv2.1 T1. (a) An electron micrograph of a Kv2.1 T1 sample negatively stained with 2%(w/v) uranyl acetate and imaged at 100 000× magnification. Inset: increased magnification to aid the visualization of individual oligomers; two examples are outlined with yellow dashed lines. (b) 2D reference-free representative class averages of particles in a top view revealed a pentameric form for Kv2.1 T1. |