Figure 6
Protein thermal unfolding in the presence of EDTA. (a) Comparison of CD spectra of Kv2.1 T1 at 10°C in the absence (black) or presence (red) of 1 mM EDTA with the calculated spectra for Kv1.1 (PDB entry 1t1d, purple), Kv3.1 (PDB entry 3kvt, blue) and Kv4.3 (PDB entry 1s1g, light blue) T1 domains. (b) Stability analysis of Kv2.1 T1 in solution using CD. The fraction of unfolding extracted from far-UV CD spectra at 222 nm was plotted as a function of temperature. (c) The hydrodynamic radius of Kv2.1 T1 monitored by dynamic light scattering is presented as a function of temperature. In (b) and (c), the black data points are for Kv2.1 T1 without EDTA and the red data points are for Kv2.1 T1 treated with 1 mM EDTA. |