Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre

Båth, P.; Banacore, A.; Börjesson, P.; Bosman, R.; Wickstrand, C.; Safari, C.; Dods, R.; Ghosh, S.; Dahl, P.; Ortolani, G.; Björg Ulfarsdottir, T.; Hammarin, G.; García Bonete, M.-J.; Vallejos, A.; Ostojić, L.; Edlund, P.; Linse, J.-B.; Andersson, R.; Nango, E.; Owada, S.; Tanaka, R.; Tono, K.; Joti, Y.; Nureki, O.; Luo, F.; James, D.; Nass, K.; Johnson, P.J.M.; Knopp, G.; Ozerov, D.; Cirelli, C.; Milne, C.; Iwata, S.; Brändén, G.; Neutze, R.

doi:10.1107/S2059798322004144

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 1
LCP microcrystallization of RC_vir. (a) Early leads after screening. (b) Thin needle-like crystals which grew in syringes and diffracted to 2.4 Å resolution at SACLA but with a low hit rate. (c) Initial crystals grown in 800 µl glass wells. These crystals diffracted to 2.2 Å resolution at SACLA but had a long crystal axis and could not be indexed. (d) Seeded LCP crystals suitable for SFX and TR-SFX studies using XFEL radiation. These crystals diffracted to 2.25 Å resolution.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 78| Part 6| June 2022| Pages 698-708

https://doi.org/10.1107/S2059798322004144

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