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Figure 3
The structure of CbpD reveals that some chitin-specific motifs are preserved while others are more similar to cellulose-specific motifs. (a) The active site of CbpD contains a canonical His-brace motif. 2Fo − Fc electron density contoured at 0.7σ allows copper to be modeled into the active site (sphere_scale 0.6), approximately 2 Å away from the three coordinating N atoms, as expected. (b) The CbpD active site also contains a number of highly conserved residues. (c) Motifs previously identified as conserved among and/or proposed to confer substrate specificity in chitin- and cellulose-active LPMOAA10s are mapped to sequence logos generated from multiple sequence alignments of 13 chitin-active and seven cellulose-active LPMOAA10s listed as experimentally validated in the CAZy database (Drula et al., 2022 ![]() ![]() ![]() ![]() ![]() |
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