Figure 4
σ-Weighted 2mFo − DFc Ric-8A electron-density maps at successive stages in the phasing procedure. 3.4 Å resolution electron-density maps computed with SAD phases from the anomalous scattering substructure corresponding to the highest ranking solution determined by SHELXD using all 18 scaled and merged data sets are shown before (a) and after (b) density modification by solvent flattening. (c) σ-Weighted mFo − DFc electron-density map computed with native data measured at wavelengths of 0.979–2.2 Å with phases calculated from the final refined model. The refined Ric-8A model is shown in stick mode (Cα atoms in dark purple); electron-density maps were contoured at 1.5σ. |