Helical-like data collection and timestamp for data sets with the corresponding temperature. (a) An enlarged view of a crystal harvested in a loop with the crystal tip slightly touching the reservoir solution. A rectangular red dashed line outlines the crystal and the bookmarked positions are illustrated in pink with the data-set numbers. (b, c) The timestamp of the data sets and the corresponding temperatures for the temperature ramp-up at 15%(v/v) DMSO and ramp-down at 5%(v/v) DMSO experiments, respectively. The blue circles and red squares represent the N2 stream temperature and the estimated actual temperature at the sample, respectively. The numbers above and below the x axis indicate the number of the data set and the time, respectively. The 10 min indicated in the figures is the equilibration time for the experiment temperature before data collection. The arrow lines in the figures are indicative and are not drawn on the scale of the real-time line.