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Figure 8
Effect of temperature on the binding of RBD proteins to human ACE2. (a) Measurement of the binding affinity of RBD wt, RBD Q498Y and RBD β at different temperatures using an ELISA-like plate assay (as described in Section 2[link]). The graphs plot the absorbance at 450 nm (y axis) for each RBD concentration in nM (x axis; logarithmic scale). Each point represents a mean absorbance value ± standard deviation (SD). Data were fitted to a saturation, one-site specific binding model built into GraphPad Prism 9.0 to obtain the corresponding curve for each RBD and the Kd values (Table 6[link]). Assays were performed in triplicate. (b) Absorbance values for RBD wt, RBD Q498Y or RBD β binding to ACE2 in a temperature-dependent manner.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 78| Part 9| September 2022| Pages 1156-1170
https://doi.org/10.1107/S2059798322007677
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