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Figure 4
PPIase activity assay. The PPIase activities of CrTIG1 and EcTF were measured with a protease-coupled assay. The prolyl cis–trans isomerization of the tetrapeptide succinyl-Ala-Phe-Pro-Phe-para-nitroanilide as a substrate was monitored by measuring the absorbance changes at 395 nm at different CrTIG1 concentrations. EcTF was used as a positive control. The inset shows the change of the rate constants depending on the CrTIG1 concentration.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 78| Part 10| October 2022| Pages 1259-1272
https://doi.org/10.1107/S2059798322009068
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