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![[Figure 1]](rr5226fig1mag.jpg)
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Figure 1
Characterization of 6H-RdfS in solution using small-angle X-ray scattering. (a) Size-exclusion chromatography profile of the 6H-RdfS monomer during the SAXS experiment. The absorbance at 260 and 280 nm is plotted against the elution volume from the Superdex 200 5/150 GL gel-filtration column. (b) Small-angle X-ray scattering profile of 6H-RdfS [log(I) versus log q]. (c) Guinier analysis of 6H-RdfS. Rg is shown adjacent to the plot line. (d) Normalized (dimensionless) Kratky plot of 6H-RdfS. The peak ![[qR_{\rm g}^{2}]](teximages/rr5226fi1.svg) is shown as a dashed line. (e) Ab initio DAMFILT-filtered bead model (based on 11 DAMMIN models) representing the elongated 6H-RdfS envelope in (i) elongated and (ii) perpendicular views. The mean iterative closest point (ICP; Besl & McKay, 1992 ) metric was 14.7 (±2.5) for all 11 DAMMIN models. (f) EOM ensemble of six RdfS cartoon models fitted into the 6H-RdfS SAXS scattering data, with the wHTH domain (black) rigid and the N-terminal helix (coloured) disordered. The various colours demonstrate the flexibility of the N-terminal helix as fitted to the scattering data (red, green, yellow, blue, purple and pink). |
![[Figure 1]](rr5226fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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