view article

Figure 1
Characterization of 6H-RdfS in solution using small-angle X-ray scattering. (a) Size-exclusion chromatography profile of the 6H-RdfS monomer during the SAXS experiment. The absorbance at 260 and 280 nm is plotted against the elution volume from the Superdex 200 5/150 GL gel-filtration column. (b) Small-angle X-ray scattering profile of 6H-RdfS [log(I) versus log q]. (c) Guinier analysis of 6H-RdfS. Rg is shown adjacent to the plot line. (d) Normalized (dimensionless) Kratky plot of 6H-RdfS. The peak [qR_{\rm g}^{2}] is shown as a dashed line. (e) Ab initio DAMFILT-filtered bead model (based on 11 DAMMIN models) representing the elongated 6H-RdfS envelope in (i) elongated and (ii) perpendicular views. The mean iterative closest point (ICP; Besl & McKay, 1992BB8) metric was 14.7 (±2.5) for all 11 DAMMIN models. (f) EOM ensemble of six RdfS cartoon models fitted into the 6H-RdfS SAXS scattering data, with the wHTH domain (black) rigid and the N-terminal helix (coloured) disordered. The various colours demonstrate the flexibility of the N-terminal helix as fitted to the scattering data (red, green, yellow, blue, purple and pink).

Journal logoSTRUCTURAL
ISSN: 2059-7983
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds