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Figure 1
Default weighting of reference-model torsion restraints in ISOLDE according to pLDDT. The φ, ψ and (if the template and model residues have the same identity) every χ dihedral in a given residue are restrained based on the pLDDT for the matching template residue; the ω dihedral is restrained to cis or trans ± 30° using the flat-bottom torsion restraints in ISOLDE. Residues where the template pLDDT is less than 50 are not restrained.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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