Figure 9
Interactive refitting of the complete model in ISOLDE. The MR solution is shown as a tan ribbon; the complete model is initialized in ISOLDE and shown as a Cα trace. The region highlighted in bright green is selected and is being pulled by the `tug selection' mouse mode in ISOLDE, with distances and torsions restrained to the AlphaFold model geometry. For this initial step the map generated by Phaser is used as the fitting potential; once the gross rearrangement is complete further rebuilding will use structure factors calculated on-the-fly from the model. |