Crystal structure of DNA polymerase I from Thermus phage G20c

Ahlqvist, J.; Linares-Pastén, J.A.; Jasilionis, A.; Welin, M.; Håkansson, M.; Svensson, L.A.; Wang, L.; Watzlawick, H.; Ævarsson, A.; Friðjónsson, Ó.H.; Hreggviðsson, G.Ó.; Ketelsen Striberny, B.; Glomsaker, E.; Lanes, O.; Al-Karadaghi, S.; Nordberg Karlsson, E.

doi:10.1107/S2059798322009895

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 1
Production and purity of PolI_G20c and ExnV1. (a) Molecular-weight and purity assessment of DNA polymerase I from Thermus phage G20c (PolI_G20c). Lane M, Precision Plus Protein Dual Colour Standards (Bio-Rad) molecular-mass marker; lane PolI_G20c, recombinant PolI_G20c purified by IMAC. (b) Production, purity and molecular-weight assessment of ExnV1. Lane M, PageRuler Unstained Broad Range Protein Ladder (Thermo Fisher Scientific); lane −, non-induced soluble crude extract of the ExnV1 expression culture; lane +, induced soluble crude extract of the ExnV1 expression culture; lane P, recombinant ExnV1 purified by IMAC.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 78| Part 11| November 2022| Pages 1384-1398

https://doi.org/10.1107/S2059798322009895

Open

access

Follow Acta Cryst. D

Search IUCr Journals		doi		Advanced search
Author		volume	page