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![[Figure 3]](cb5141fig3mag.jpg)
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Figure 3
Probing AAR2Δloop–PRPF8–SNRNP200 interactions and AAR2 phosphorylation. (a–d) SDS–PAGE analyses (left) and UV elution profiles (right) of analytical size-exclusion chromatography runs monitoring the interactions among AAR2, PRPF8RH-JM and SNRNP200395–2136 (a, b) and among AAR2, PRPF8RH, PRPF8JM and SNRNP200395–2136 (c, d). (e) Close-up view of the region in AAR2Δloop–PRPF8RH surrounding Ser284 of AAR2Δloop. The corresponding region in yeast Aar2p is profoundly restructured upon replacement of the equivalent Ser253 by a phosphomimetic glutamate residue (Weber et al., 2013  ). (f) SDS–PAGE analysis (top) and UV elution profile (bottom) of an analytical size-exclusion chromatography run monitoring the interaction between AAR2S284E and PRPF8RH. In panels showing SDS–PAGE gels and elution profiles lane M contains molecular-mass standard (kDa) and lane I contains input samples. Protein bands are identified on the right. Elution fractions are indicated at the top of the gels and profiles; elution volumes are indicated at the bottom of the profiles. Icons are explained at the bottom. Variants are indicated below the respective icons. Peaks labeled by transparent icons represent an excess of the respective protein. |
![[Figure 3]](cb5141fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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