journal menu
![[Figure 3]](di5057fig3mag.jpg)
|
|
Figure 3
Determination of the activity of CapGH_57. (a) Activity of CapGH5_57 obtained by the DNS method against different types of substrates including mannans, glucomannans, galactomannans and galactoglucomannans. LBG, HV, MV and LV correspond to locust bean gum, high viscosity, medium viscosity and low viscosity, respectively. (b) Reaction of CapGH5_57 with deacetylated konjac glucomannan (KGM) evaluated by mass spectrometry. DP corresponds to the degree of polymerization of the detected oligosaccharide and ranges from 2 to 6. The chromatographic method allowed the discriminatation of different isomers of DP2. However, the obtained chromatographic resolution at the retention times corresponding to DP3, DP4, DP5 and DP6 did not resolve isomers within the same degree of polymerization. (c) 20% PACE gel of CapGH5_57 reaction products on deacetylated KGM. The arrows indicate the products generated by CapGH5_57 on deacetylated KGM. The background bands from KGM and CapGH5_57 support the new bands obtained in `CapGH5_57 + KGM' as products of the enzymatic reaction. |
![[Figure 3]](di5057fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
