Tomograms of human 80S ribosome vitrified on a regular holey carbon grid and an ssDNA-coated grid. Samples of human 80S ribosome vitrified on regular (a–c) and ssDNA-coated (d–f) grids. (a, d) XZ planes from tomograms with the upper and lower air–water interfaces indicated by white and black triangles with dotted lines of the same colors, respectively. (b, e) XY planes from the top sections of the tomograms. (c, f) XY planes from the bottom sections of the tomograms. The white and black triangles and dotted lines in (b)–(f) indicated the positions of the cross-sections shown in (a) and (d).