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Figure 3
Active-site cleft details and proposed activation mechanism of pLAST. (a) Close-up view of Fig. 2[link](a) in stereo depicting residues engaged in the PP–CD interaction as sticks with C atoms in green (PP; blue labels) or plum (CD; red labels). The labels of the residues shown in Fig. 2[link](c) have not been included for clarity. (b) Superposition in stereo of the Cα traces of the experimental structure of pLAST (in tan for the CD moiety and semi-transparent aquamarine for the PP) and the AlphaFold homology model of LAST (in salmon) to illustrate the proposed activation mechanism. Small differences are found in segments G199–D206 (1) and E150–E179 (2) owing to a closing motion that slightly narrows the cleft. Large differences are encountered for the `activation segment' (3; P180–N187) and the first seven residues of the mature CD (4; N49–L56). Green arrows pinpoint the proposed movements upon maturation.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 78| Part 11| November 2022| Pages 1347-1357
https://doi.org/10.1107/S2059798322009688
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