Structural insight into the hydrolase and synthase activities of an alkaline α-galactosidase from Arabidopsis from complexes with substrate/product

Chuankhayan, P.; Lee, R.-H.; Guan, H.-H.; Lin, C.-C.; Chen, N.-C.; Huang, Y.-C.; Yoshimura, M.; Nakagawa, A.; Chen, C.-J.

doi:10.1107/S2059798323000037

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 8
Active-site structures of D383A in complex with raffinose. The structures of D383A in complexes with raffinose (magenta), with stachyose (green) and with hydrolyzed raffinose, i.e. galactose and sucrose (cyan), in the catalytic binding pocket shown with the electrostatic surface indicate that the wider binding-site pocket of the alkaline α-galactosidase AtAkαGal3 provides a new secondary product-binding site to stabilize the sucrose moiety (cyan).

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 2| February 2023| Pages 154-167

https://doi.org/10.1107/S2059798323000037

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