Structural insight into the hydrolase and synthase activities of an alkaline α-galactosidase from Arabidopsis from complexes with substrate/product

Chuankhayan, P.; Lee, R.-H.; Guan, H.-H.; Lin, C.-C.; Chen, N.-C.; Huang, Y.-C.; Yoshimura, M.; Nakagawa, A.; Chen, C.-J.

doi:10.1107/S2059798323000037

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 9
The active site for galactose and the secondary active site for sucrose. (a) A side view of the superimposed substrate raffinose (magenta) and products galactose and sucrose (cyan) from hydrolyzed raffinose in the catalytic binding pocket shown as an electrostatic surface. An empty space is found when the substrate raffinose is bound; this space is then occupied by the sucrose product after raffinose has been hydrolyzed. (b) A top view clearly shows that after raffinose has been hydrolyzed the sucrose product is flipped 180° and occupies the secondary product-binding site.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 2| February 2023| Pages 154-167

https://doi.org/10.1107/S2059798323000037

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