March 2023 issue
The crystallization facility at Hauptman–Woodward Medical Research Institute, now the National High-Throughput Crystallization Center, has served the structural biology community for over two decades. The lessons learned from our high-throughput crystallization services, current operations and capabilities are described, together with a vision for the future of crystallization.
Errors from measurement, data processing and modelling are present throughout structures deposited in the Protein Data Bank. Identifying them is only the first step. What needs to change in order to minimize their impact?
Anomalous information was obtained from model lysozyme, thaumatin and proteinase K crystals from 220 K up to physiological temperatures.
Correction of serial crystallography diffraction intensities for lattice-translocation defects enabled the interpretation of time-resolved serial femtosecond crystallography data probing the first molecular motions in vertebrate vision.
AlphaFold predictions can be used both as a starting point for structure determination and as a method of model optimization. The Phenix PredictAndBuild tool automates iterative prediction and model building, yielding a density map and model starting with sequence information and crystallographic data.
YdaT, a functional equivalent of the bacteriophage λ CII repressor in certain lambdoid phages, is a member of the POU-domain family and recognizes a 5′-TTGATTN6AATCAA-3′ inverted repeat.
The tetramerization domain of the bacterial gene repressor Rco from Bacillus subtilis plasmid pLS20 was identified. The structure shows high similarity to the tetramerization domain of the p53 family of human tumor suppressors, despite having low sequence homology.