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Figure 4
Native mass spectrometry. (a) Native MS spectrum of YdaT at a concentration of 2.5 µM (tetramer concentration). Next to the expected tetramer, peaks corresponding to a YdaT monomer are also visible. (b) When an excess amount of the 30 bp OM operator fragment (15 µM of duplex) is added to YdaT (2.5 µM of tetramer), a single species of a YdaT tetramer bound to two OM duplexes is observed next to an excess of free DNA. (c) When the OM DNA duplex (1.67 µM) becomes saturated with YdaT (2.5 µM), the dominant species becomes a YdaT tetramer bound to a single OM duplex.

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ISSN: 2059-7983
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