Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7

Prolič-Kalinšek, M.; Volkov, A.N.; Hadži, S.; Van Dyck, J.; Bervoets, I.; Charlier, D.; Loris, R.

doi:10.1107/S2059798323001249

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 6
EMSA. (a) EMSA experiments performed using increasing concentrations of YdaT tetramers on either a 206 bp fragment of the ydaT promotor/operator region or a similar sized fragment of the Cupriavidus metallidurans prsQ2 promotor/operator region. (b) EMSA of YdaT^1–96 on the 206 bp ydaT promotor/operator DNA fragment showing a lack of binding.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 3| March 2023| Pages 245-258

https://doi.org/10.1107/S2059798323001249

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