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Figure 5
Yeast α-mannan hydrolysis by NnGH92. (a) The hydrolysates were analysed at three different time points using the reducing-sugar assay (PAHBAH). The degree of conversion was calculated based on the total mannose released after strong acid hydrolysis (see Section 2[link]). (b) Product release pattern analysed by TLC. Lane 1 represents standards composed of β-1,4-mannooligosaccharides with a degree of polymerization (DP) between 1 and 6. Lanes 2 and 3 correspond to the α-mannan hydrolysates after 1 h at enzyme concentrations of 0.1 and 1 µM. Only a single band was observed, corresponding to DP1.

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