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Figure 6
Activity and binding profiles of the NnGH92 variants. (a) Yeast α-mannan hydrolysis. The variants at different enzyme concentrations were mixed with 2.5 g l−1 yeast α-mannan and incubated for 1 h at 37°C. The activity was calculated based on the release of the reducing sugar ends (PAHBAH). (b) The variants binding to yeast cell-wall extracts (see Section 2[link]). No binding was found for the ΔN-CBM and core variants and control (BSA). Solid lines represent the fitted Langmuir equation. Error bars represent standard deviations from triplicate measurements.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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