Conformational transition of the Ixodes ricinus salivary serpin Iripin-4

Kascakova, B.; Kotal, J.; Havlickova, P.; Vopatkova, V.; Prudnikova, T.; Grinkevich, P.; Kuty, M.; Chmelar, J.; Kuta Smatanova, I.

doi:10.1107/S2059798323002322

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 1
Analysis of heat-stable complex formation between Iripin-4 and tested proteases. No complex formation was observed. There is visible cleavage of Iripin-4 by porcine elastase, as shown by a black arrow. Proteins were resolved on 12% NuPAGE Bis-Tris gels and visualized by Coomassie staining.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 5| May 2023| Pages 409-419

https://doi.org/10.1107/S2059798323002322

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