Near-atomic resolution reconstructions from in situ revitrified cryo samples

Bongiovanni, G.; Harder, O.F.; Voss, J.M.; Drabbels, M.; Lorenz, U.J.

doi:10.1107/S2059798323003431

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 1
Illustration of the workflow used to obtain a near-atomic resolution reconstruction from in situ revitrified cryo samples. (a, b) Cryo samples on UltrAuFoil grids are melted and revitrified in situ with a modified Jeol 2200F transmission electron microscope. (c) The samples are then transferred to a Titan Krios G4 for high-resolution imaging. (d) Single-particle reconstructions from the revitrified areas yield near-atomic resolution maps.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 6| June 2023| Pages 473-478

https://doi.org/10.1107/S2059798323003431

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