Figure 3
Revitrification reshuffles the particles, which results in a more homogeneous angular distribution. (a) Relative precision of the reconstructions at 1.9 Å as a function of angle in the conventional (left) and revitrified (right) sample areas with octahedral symmetry applied. The relative precision reported by cryoSPARC approximately reflects the number of observations contributing information to a specific angle. (b) A micrograph of a conventional sample area shows a homogeneous distribution of the particles (left), whereas an uneven particle distribution is observed in revitrified areas (right). |